Sulfated alginate as a mimic of sulfated glycosaminoglycans: Binding of growth factors and effect on stem cell behavior

last updated: 2017-06-19
ProjectCHEM2NATURE :: publications list
TitleSulfated alginate as a mimic of sulfated glycosaminoglycans: Binding of growth factors and effect on stem cell behavior
Publication TypePapers in Scientific Journals
Year of Publication2017
AuthorsMhanna R., Becher J., Schnabelrauch M., Reis R. L., and Pashkuleva I.
Abstract

Sulfated glycosaminoglycans (GAGs) are principal elements of the extracellular matrix, where they are involved in a plethora of signaling pathways mainly via interactions with diverse proteins such as growth factors and cytokines. However, the mechanisms that drive these interactions are not yet clear, mostly
because of the difficulty to access large quantities of homogeneously sulfated natural GAGs. In this work, GAG mimics are synthesized from readily available alginate with different degrees of sulfation (DS, from 0.8 to 2.6) by simple process. The effect of the DS is determined on the binding of basic fibroblast growth factor (FGF-2). The enzyme-linked immunosorbent assay demonstrates that the binding of FGF-2 is significantly greater for alginates with high DS as compared to unmodified and low sulfated analogs. These results are
further applied to engineer FGF-2-loaded substrates for stem cell culturing using the layer-by-layer approach. All films support the attachment and growth of adipose derived stem cells (ADSCs). Noteworthy, highly sulfated alginates maintain the stemness of the ADSCs that exhibit remarkably long filopodia. These results can be exploited in the engineering of novel substrates that induce targeted cell behavior via controlled protein delivery and for tissue engineering constructs applicable in various regenerative approaches.

JournalAdvanced Biosystems
Volume1
Date Published2017-06-16
PublisherWiley
ISSN2366-7478
DOI10.1002/adbi.201700043
URLhttp://onlinelibrary.wiley.com/doi/10.1002/adbi.201700043/epdf
KeywordsALGINATE, biomimetic materials, growth factors, layer-by-layer, sulfated glycosaminoglycans
RightsrestrictedAccess
Peer reviewedyes
Statuspublished

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